Emergence of Salmonella Senftenberg in animal feeds in Ukraine: Antimicrobial resistance and biofilm formation

Authors: Natalia Shchur, Maksym Shevchenko, Liudmila Shalimova, Iryna Musiiets, Maksym Karpulenko, Leonid Korniienko, Vitalii Ukhovskyi, Nataliia Kuriata, Oleksandr Pishchanskyi, Andrii Andriichuk, Taras Tsarenko, and Vitalii Nedosekov

Ger. J. Microbiol. 2025. vol. 5, Iss. 2 pp:13-26
Doi: https://doi.org/10.51585/gjm.2025.2.0050

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Abstract:

In this study, Salmonella enterica serovar Senftenberg was isolated from plant-based animal feeds for the first time in Ukraine. This serovar is associated with human salmonellosis, which is caused by consuming contaminated poultry products and plant-derived foods, underscoring its zoonotic and safety relevance. The studied isolates were isolated at the same enterprise in two different seasons (autumn and spring) over a two-year period, indicating a stable source of contamination and characterizing the serovar as resistant to aggressive environmental factors. The isolates were susceptible to 84% (16/19) of antibiotics, exhibiting an atypical resistance profile characterized by selective resistance to cefoxitin (with sensitivity to other cephalosporins) and imipenem (with sensitivity to meropenem). The highest biofilm biomass was recorded at 37°C with pronounced inter-isolate differences. Two isolates demonstrated stable weak biofilm formation, and the third showed minimal activity. This work includes preliminary studies on Salmonella enterica serovar Senftenberg's ability to form biofilms and preserve viable cells in biofilms. This is the first study of Salmonella enterica serovar Senftenberg in Ukraine, highlighting the need to strengthen epidemiological surveillance of emerging serovars in feed production and develop specific control strategies based on the identified resistance and biofilm formation characteristics.

Keywords:

Zoonotic pathogen, Serovar Senftenberg, Plant-based feed, Antimicrobial resistance, Biofilm formation

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